Journal: Scientific Reports

Article Title: In vitro exercise model using contractile human and mouse hybrid myotubes

doi: 10.1038/s41598-019-48316-9

Figure Lengend Snippet: Contraction-inducible upregulation of the expressions and secretions of myokines. After 7–8 days of differentiation, the differentiated myotubes originating from human samples alone (HSMM), human-origin and mouse-origin cells in a mixture (HSMM + C2C12), and murine C2C12 cells alone were treated with or without EPS at 20 V/25 mm, 1 Hz, 4-ms duration for 16 h. ( A ) Total RNA was extracted and the relative abundances of mRNAs for mouse IL-6 and CXCL1 as well as human IL-6 and CXCL1 were evaluated by real-time PCR analysis. Data normalized using mouse GAPDH or human RPLP0 transcripts were averaged over 3 independent experiments ( *P < 0.05). ( B ) Conditioned media were collected and concentrations of human IL-6 and CXCL1 as well as mouse IL-6 and CXCL1 were evaluated employing the BioPlex assay ( *P < 0.05, n = 3).

Article Snippet: The mouse CXCL1 and IL-6 concentrations were measured employing a house-made BioPlex assay with antibodies from the commercially available ELISA kits (R & D Systems, Minneapolis, MN).

Techniques: Real-time Polymerase Chain Reaction, BioPlex Assay

Journal: eLife

Article Title: Overriding impaired FPR chemotaxis signaling in diabetic neutrophil stimulates infection control in murine diabetic wound

doi: 10.7554/eLife.72071

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , Recombinant Mouse CXCL1/KC Protein , R & D Systems , Cat# 453-KC , .

Techniques: Control, Staining, Sequencing, Enzyme-linked Immunosorbent Assay, Competitive ELISA, Recombinant, Software, SYBR Green Assay

Journal: Immunity

Article Title: Dietary Fiber Confers Protection against Flu by Shaping Ly6c - Patrolling Monocyte Hematopoiesis and CD8 + T Cell Metabolism.

doi: 10.1016/j.immuni.2018.04.022

Figure Lengend Snippet: Figure 3. Dietary Fiber Prevents Neutrophil Influx into the Airways in a SCFA- and FFAR3-Dependent Manner by Reducing Macrophage- Derived CXCL1 Production (A) Viral load in the lung of mice fed a control or HFD 1, 3, and 5 days after low-dose infection as measured by viral RNA expression in relation to bActin. (B) Neutrophil counts in the BALF of mice fed a control or HFD 1, 3, 5, and 8 days after low-dose infection as determined by differential cell counts. (C) Differential cell counts in the BALF of mice treated with butyrate 5 days after low-dose infection. Mac, macrophages; Neu, neutrophils; Eos, eosinophils; Lym, lymphocytes. (D) Differential cell counts in the BALF of wild-type, Ffar2/, and Ffar3/ mice fed a control or HFD 5 days after infection. Mac, macrophages; Neu, neutrophils; Eos, eosinophils; Lym, lymphocytes. (E) Experimental model of neutrophil reduction during high-dose infection in butyrate-treated animals. (F) Survival (p = 0.0201 for water/isotype versus butyrate/isotype; p = 0.0042 for water/isotype versus water/aLy6G) and clinical score of neutrophil-reduced mice receiving either nothing or butyrate in drinking water. (G) Frequency of granulocyte-macrophage progenitors (GMPs) in the bone marrow of control or HFD-fed mice 5 days after low-dose infection. (H) Frequency of neutrophils in the bone marrow and in the blood of control or HFD-fed mice 5 days after low-dose infection. (I) Frequency of neutrophils in the BALF of mice fed a control or HFD 6 hr after intranasal CXCL1 administration. (J) mRNA expression of colony-stimulating factor 3 (Csf3) in the lung of mice fed a control or HFD 5 days after low-dose infection. (K) Protein content of CXCL1 in the BALF of control or HFD-fed mice 3 and 5 days after low-dose infection. (L) CXCL1 expression in the CD45 compartment, neutrophils, and monocytes/macrophages in control and HFD-fed animals 3 days after low-dose infection as determined by flow cytometry. MFI, mean fluorescence intensity.

Article Snippet: CXCL1 intracellular expression was assessed after 4h incubation with Brefeldin A (Biolegend) using an unlabelled primary antibody against murine CXCL1 (R&D Systems, MAB4532, 1:100) and a goat-anti-rabbit-PE secondary antibody (R&D Systems, F0110, 1:50) to visualize the signal.

Techniques: Derivative Assay, Control, Infection, RNA Expression, Expressing, Cytometry